Facility Cores

 

Integrative Health Sciences Facility Core

Director: William Rom, M.D., Professor, Departments of Medicine and Environmental Medicine
Co-Directors: Qingshan Qu, MD., Associate Professor, Department of Environmental Medicine
Yu Chen, Ph.D., Assistant Professor, Department of Environmental Medicine

 

1. Overview

The Integrative Health Sciences Facility Core (IHSFC) was initially organized in November, 2007 and is now composed of four sub service cores and one well-developed international partnership. The overall mission of our IHFSC is to promote the use of human samples by bench researchers. The NYU Center has a plethora of outstanding basic science research, and our vision, with the active participation of the IHSFC, is to translate our basic research results into human health studies. Since its inception about three years ago, the IHSFC has been very active in implementing this new vision for the Center, expanding on the knowledge gained from prior research using cell culture or animal models. Another goal of this facility core is to promote interactions and productive collaborations between Center investigators with research focusing on mechanistic studies of environmental health hazards and Center members involved in clinical and population-based epidemiologic studies. To achieve this goal, the IHSFC has been providing and will continue to offer comprehensive services to all Center investigators who are planning to conduct translational research through its 4 sub facility cores and the international partner, including: 1) environmental epidemiology services; 2) ethical issues and QC/QA support; 3) exposure sampling and assessment; 4) clinical facility for research of environmental hazards; and 5) Lanzhou University School of Public Health (See Figure below).

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2. Services Provided According to the Sub-Facility Cores

Environmental Epidemiology Services

The leadership of this sub-core has substantial experience in observational epidemiologic studies with a focus on environmental exposure. Services provided include the following:
• Consultation for appropriate study design (case-control, case-cohort, cohort, or cross-sectional) and related methodology issues.
• Assistance on epidemiology questionnaire design for new studies that will capture key confounding factors, risk factors for disease of interest, and exposure information.
• Referrals to the Environmental Health Statistics and Bioinformatics core for statistics and bioinformatics, which would include management of high throughput data such as genomics, ChIP-seq, etc.
• Identification of appropriate and existing study populations.
• Referrals to appropriate epidemiologists as potential collaborators with basic scientists to promote interdisciplinary projects and translational studies.
• Consultation on the methods and pilot studies to evaluate reproducibility and validity of the exposure and effect measures including biomarkers of disease and environmental exposures.
Ethical Issues and QC/QA Support
The leadership and personnel of this sub facility core have extensive experience in human population research, and therefore, will be able to assist Center investigators directly or to make referrals to other Center members for assistance and collaborations. The following specific services are provided:
• Assistance in the preparation of Human Subject Protocols, Consent Forms, and Institutional Review Board (IRB) applications.
• Consultation for QA and QC procedures in biomarker measurements and human population studies.
• Consultation for methods related to sample collection, processing, storage, and labeling.
• Assistance in maintenance of archived biological samples in the Center and with active searches to identify existing well-characterized populations for Center members.
Exposure Sampling and Assessment
In order to provide services outlined below, this sub core of the IHSFC is structured with expertise in environmental exposure monitoring and equipped with both ambient and personal exposure sampling devices and analytical instrument. The leadership of this sub core has expertise to effectively support Center investigators' research activities in relation to:
• Ambient and personal exposure sampling and monitoring
• Analysis for pollutants of interest in the collected samplers
Clinical Facility for Environmental Research
The leadership and personnel related to this sub-core will provide the following services to the Center investigators:
• Consultation for studies that aim to identify and validate biomarkers for the early detection of lung cancer.
• Consultation for studies that investigate the interaction between genetic susceptibility and smoking and asbestos exposure in lung cancer risk or biomarkers for lung cancer.
• Assistance to identify a clinical epidemiologist or a physician that can work with a basic scientist in recruiting new participants or using existing samples collected at the NYU Lung Cancer Biomarker Center.
• Clinical services to the Center investigators whose research projects require special clinical examination or laboratory tests related to lung diseases for his/her study subjects.

 

3. Existing Biological Samples that can be used for New Collaborations

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4. Operational Policies

In general, the resources and services of the Facility Core will be made available to all faculty and staff in the Department and other schools, however, Center members are given priority over non-members. Furthermore, among all potential research projects, the Center Pilot Projects planning to collect human preliminary data for grant applications are given a special priority for consideration.
Request for using existing biospecimens to develop preliminary results needs to be first approved by and collaborated with the original source PIs. The director and Co-directors of the Core will be responsible for assuring that all initiated collaborations comply with all applicable federal regulations and IRB policies.

CONTACT INFORMATION

Qingshan Qu, MD., Associate Professor
Phone: (845)731-3567
Email: qingshan.qu@nyumc.org
Yu Chen, Ph.D., Assistant Professor
Phone: (212)263-4839
Email: yu.chen@nyumc.org

 

 

Specialized Inhalation Exposure Facility

Director: Lung Chi Chen, Ph.D.

NYU's Department of Environmental Medicine has one of the largest, if not the largest, academic inhalation exposure facilities in the U.S. Developed in the 1960s by Professor Sidney Laskin, the inhalation exposure facility is composed of both large and small inhalation exposure chambers and state-of-the-art exposure monitoring equipment. An NIH reconstruction grant recently supported the incorporation of several inhalation chambers directly into the animal housing facility. The exposure facility is utilized by a variety of NYU investigators as well as researchers from other Universities (on a cost-fee basis).

INHALATION EXPOSURE UNIT

• 27 dynamic, stainless steel, glass, and Plexiglas Laskin-type inhalation exposure chambers, including 4 glove-box type units;
• 7 small Plexiglass exposure chambers;
• Specialized glove-box isolator housing for carcinogens or infectious agent exposures
• Completely equipped combustion furnaces (250 sq ft), and particle concentrator facility;
• Supplementary calibration facilities and sampling equipments with the following analytical chemistry equipments: 2 gas chromatographs, 2 UV-visible spectrophotometers, 4 infrared gas analyzers, several ozone and sulfuric and nitric acid analyzers, and particle measurement equipment;
• An array of specialized equipments for the generation of highly standardized gaseous, vapor, and particulate inhalation atmospheres of different composition, including cigarette-smoke generators (for both main-stream and side-stream smoke).

CONTACT INFORMATION:

For availability of services, and fees, please contact,
Lung Chi Chen, Ph.D.
NYU Langone School of Medicine
Sterling Forest Campus
Phone: 845-731-3510
FAX: 845-351-4015
lung-chi.chen@nyumc.org

 

 

MOLECULAR CELL and ANALYTICAL SERVICES FACILITY CORE

Director: Catherine B. Klein, Ph.D.

Co-Director: Isaac Wirgin, Ph.D.

The Molecular Cell and Analytical Services (MCASFC) Facility Core provides NIEHS Center members with education on, and access to, state-of-the-art tools for assessing the effects of the environment on human and mammalian genomes and epigenomes. The Core also provides analytical services, particularly those for metals analysis in cells and tissues of exposed organisms, since metal effects on the genome and epigenome are a specialized research strength of the NYU Center. This Facility Core supports training and methods development on the use of molecular and cell biology and metals analysis shared equipment under an administrative umbrella that facilitates optimal use of state-of-the-art shared equipment. Furthermore, the MCASFC provides Center investigators with evolving access to specialized methodologies and equipment, either on-site or off-site, by taking advantage of regional resources involving intra- and inter-university research partnerships. In 2009, this Facility Core provided equipment, supplies, "hands on" training and intellectual expertise to 18 NIEHS Center investigators and their research support staff, students, and post-doctoral trainees. The Facility Core provides value-added benefits, which include fostering collaborations among investigators in different programmatic research tracks, often leading to the generation of new preliminary data, pilot studies and fully funded collaborative projects.

SOME SERVICES and EQUIPMENT AVAILABLE VIA THE MCASFC:

• ChIP-Seq and RNA-Seq
• Yeast Screen
• ABI 7900 HT RT-PCR
• ABI 7300 RT-PCR
• Beckman Coulter Biomek 2000 Laboratory Automation Workstation
• PSQ HS 96 DNA PyrosequencerTM
• Beckman Coulter CEQ8000 Automated DNA Sequencer
• Affymetrix GeneChip
• Nanodrop ND-1000
• Molecular Dynamics Storm 860 Phosphorimager
• Nanodrop NN-3300
• MACSQuant Flow Cytometer
• Beckman Coulter Epics XL-MCL Flow Cytometer
• Beckman Coulter Counter Model Z2
• FRET Imaging System
• Ciphergen ProteinChip® System Series 4000 SELDI-TOF-MS
• Solaar Atomic Absorption system (Model M6)
• XRF (Jordan Valley)

CONTACT INFORMATION:

For availability of services, fees, and to schedule training or instrument use please contact,
Catherine B. Klein, Ph.D.
NYU Langone School of Medicine
Sterling Forest Campus
Phone: 845-731-3510
FAX: 845-351-4015
Catherine.klein@nyumc.org

Isaac Wirgin, Ph.D.
NYU Langone School of Medicine
Sterling Forest Campus
Phone: 845-731-3548
FAX: 845-531-5472
Isaac.wirgin@nyumc.org